Topic 2.3
Modern cryopreservation procedures
Most people treat cryopreservation as a binary choice: sign up or don't. But execution quality matters enormously, and you can't evaluate quality without understanding the.
First response (Standby & Stabilization)What happens after legal death: standby, cooling, cardiopulmonary support, medicines, measurement and the handoff to cryoprotective perfusion.Surgical procedure (perfusion & cryoprotection)How vascular access, blood washout and graded cryoprotectant perfusion balance ice suppression against toxicity, osmotic stress and uneven distribution.Logistics (Red tape & transportation)Tomorrow.bio and local funeral partners handle the paperwork, permits and transport to Switzerland. Once documents are complete, ground and air transfer are established logistics.Biostasis quality check proceduresCryopreservation quality requires complementary evidence: case timing, perfusion telemetry, standardized whole-body CT, electron microscopy of consented neural samples, controlled.Cooldown to cryogenic temperaturesTomorrow.bio uses computer-controlled cooling, multiple internal thermocouples and a staged protocol: rapid descent near the glass transition, stress-relieving annealing, then.How vitrification procedures developed over timeVitrification developed through linked advances in cryoprotectant chemistry, stepwise perfusion, ice control, thermal engineering, organ recovery, volumetric rewarming and direct.Long-term storage facilityEBF’s Rafz facility combines passive liquid-nitrogen storage with controlled cooldown, cryogenic CT, research infrastructure, separate patient funding, Swiss foundation governance.Classic Cryogenic Storage SystemHow passive liquid-nitrogen storage combines vacuum-insulated dewars, weekly replenishment, continuous monitoring, redundant automated refilling, remote supervision and human.Modern Cryogenic Storage System (ITS)Tomorrow.bio has received the world's first human-sized ITS dewar. It is now undergoing multi-month testing of thermal performance, nitrogen consumption and failure behaviour.Evaluating ischemic exposure in cryonics (S-MIX metric)How S-MIX converts a time, temperature and support history into estimated normothermic-equivalent ischaemic exposure, including its calculation, assumptions, uses and limitations.